Molecular probes for plant cell wall polymers

PlantProbes is a distribution service for monoclonal antibodies and molecular probes directed to plant cell wall components generated by the Paul Knox Cell Wall Lab at the University of Leeds. Funds received are used to maintain antibody stocks and antibody availability.

Our cell wall antibodies are highly sensitive, highly specific and versatile molecular tools that can be used for microscopy analyses and quantitative assessments of cell wall polymers in all plant materials ranging from fruits and vegetable to fibres and all forms of plant biomass. We are happy to discuss any potential uses or applications of our probes.

Contact & Ordering Information

LM15 xyloglucan / tomato fruit cell LM5 galactan / carrot root apex LM19 homogalacturonan / Arabidopsis seed endosperm LM20 homogalacturonan / Miscanthus stem LM7 homogalacturonan / intercellular spaces LM8 xylogalacturonan / Arabidopsis root apex LM11 xylan / Arabidopsis stem LM7 homogalacturonan / tomato fruit cell LM13 arabinan / stomatal cell walls LM15 xyloglucan / pea stem vascular bundle LM16 rhamnogalacturonan-I / tobacco stem vascular cells

 

PlantProbes is based in Leeds in the UK. All enquiries & requests for monoclonal antibodies should be by email or fax. To order, please let us know the items required, delivery and invoice addresses and a purchase order number if appropriate. Items can be paid for credit card (preferred) or by institutional invoice - ask for details. Please note that PlantProbes may run a reduced service during July and August.

Download the PlantProbes catalogue

Email: antibodies@plantprobes.net
FAX: +44-(0)113-3433144

Rat monoclonal antibodies can be supplied as 1 ml or 5 ml units of hybridoma cell culture supernatants. Select any combination of rat monoclonal antibodies and see the table below for costs & discounts for multiple purchases.

Costs in UK£ (GBP)

# units

1 ml

5 ml

1

130

280

2

240

500

3

320

680

4

390

800

5

450

900

 

Antibodies are generally sent by standard airmail. A shipping and handling charge of 30 GBP will be added to all orders. Express shipment by courier will cost an additional 50 - 80 GBP. If required please specify this with the order details.

Our rat hybridoma cell culture supernatants contain sodium azide as a preservative, are shipped at ambient temperature and should be stored at 4°C.

NEW: Purified rat Mabs: LM5 galactan, LM6 arabinan, LM10 xylan and LM15 xyloglucan are now also available in 50 µg aliquots of purified immunoglubulins. Purified rat Mabs cost 220 GBP per 50 µg unit.

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Products

RAT hybridoma cell culture supernatants

Hemicellulose probes view »

Pectin probes view »

Glycoprotein probes view »

Other probes view »

HIS-tagged recombinant proteins

PAM1  Anti-homogalacturonan scFv

CBM3a Crystalline cellulose-directed CBM

MOUSE hybridoma cell culture supernatant

2F4   Anti-homogalacturonan

The JIM- series of rat monoclonal antibodies

PlantProbes can supply hybridoma supernatants for a range of JIM-designated anti-arabinogalactan-protein (AGP) and anti-extensin rat monoclonal antibodies.
These include:

anti-AGP: JIM4, JIM8, JIM13, JIM14, JIM15 & JIM16,
anti-extensin: JIM11, JIM12, JIM19 & JIM20

These rat monoclonal antibodies are available in 1 mL or 5 mL units of hybridoma supernatant.

Links to useful/related sites

Knox Cell Wall Lab at plantcellwalls.net
Activities and outputs from the Paul Knox lab that generates the LM- series of cell wall monoclonal antibodies.

CarboSource Services
The CCRC can also supply JIM5, JIM7 and a range of related cell wall monoclonal antibodies.

Agrisera
Primary antibodies for plant and algal proteomics.

NZYTech
Genes and enzymes relating to cell wall carbohydrates.

Cat. No. LM10

MONOCLONAL ANTIBODY to XYLAN / LM10

(Rat IgG2c)

SPECIFICITY: Generated using a neoglycoprotein (xylopentaose-BSA). It can recognise unsubstituted and relatively low-substituted xylans in several species. It has no cross-reactivity with wheat arabinoxylan.

REFERENCE: McCartney et al. (2005) J. Histochem. Cytochem 53, 543-546. ONLINE

Cat. No. LM11

MONOCLONAL ANTIBODY to XYLAN / ARABINOXYLAN / LM11

(Rat IgM)

SPECIFICITY: Generated using a neoglycoprotein (xylopentaose-BSA). It can recognise unsubstituted and relatively low-substituted xylans in several species. It can also accommodate more extensive substitution of a xylan backbone and binds strongly to wheat arabinoxylan. 

REFERENCE: McCartney et al. (2005) J. Histochem. Cytochem 53, 543-546. ONLINE

Cat. No. LM27

MONOCLONAL ANTIBODY to GRASS XYLAN PREPRATIONS/ LM27

(Rat IgM)

SPECIFICITY: Generated using a complex pectic immunogen. Epitope is unknown but LM27 binds strongly to preparations of grass xylan/glucuronoxylan and is likely to be an epitope of a complex substitution of grass heteroxylan or an associated molecule.  

REFERENCE: Cornuault et al. (2015) Planta 242, 1321-1334. ONLINE

Cat. No. LM28

MONOCLONAL ANTIBODY to GLUCURONOXYLAN/ LM28

(Rat IgM)

SPECIFICITY: Generated using a complex pectic immunogen. The monoclonal antibody can recognise glucuronosyl substituted xylans in several species and MeGlcA is not required for recognition.

REFERENCE: Cornuault et al. (2015) Planta 242, 1321-1334. ONLINE

Cat. No. LM15

MONOCLONAL ANTIBODY to XYLOGLUCAN / LM15 

(Rat IgG2c)

SPECIFICITY: Generated using a neoglycoprotein incorporating the xylosylated heptasaccharide from tamarind xyloglucan (XXXG-BSA). It recognises the XXXG motif of xyloglucan in several species and can accommodate to some extent a single galactosyl residue. 

REFERENCE: Marcus et al. (2008) BMC Plant Biology 8, 60. ONLINE

Cat. No. LM24

MONOCLONAL ANTIBODY to XYLOGLUCAN / LM24

(Rat IgG2a)

SPECIFICITY: Generated using a neoglycoprotein incorporating a mixture of galactosylated oligosaccharides from tamarind xyloglucan (XXLG/XLLG-BSA). In oligosaccharide microarray assays LM24 binds preferentially to the XLLG motif of xyloglucan.  It can bind to xyloglucan polymers in several species.      

REFERENCE: Pedersen et al. (2012) J. Biol. Chem. 287, 39429-39438 ONLINE

Cat. No. LM25

MONOCLONAL ANTIBODY to XYLOGLUCAN / LM25

(Rat IgGM)

SPECIFICITY: Generated using a neoglycoprotein incorporating a mixture of galactosylated oligosaccharides from tamarind xyloglucan (XXLG/XLLG-BSA). In oligosaccharide microarray assays LM25 binds to the XLLG, XXLG and XXXG oligosaccharides of xyloglucan. It can bind to xyloglucan polymers in several species .      

REFERENCE: Pedersen et al. (2012) J. Biol. Chem. 287, 39429-39438 ONLINE

Cat. No. LM21

MONOCLONAL ANTIBODY to MANNAN / LM21 

(Rat IgM)

SPECIFICITY: This antibody recognises β-linked mannan polysaccharides of plant cell walls. It has no known cross-reactivity with other polymers and can recognise heteromannan polysaccharides in several species. LM21 binds effectively to β-(1→4)-manno-oligosaccharides from DP2 to DP5. LM21 displays a wide recognition of mannan, glucomannan and galactomannan polysaccharides.

REFERENCE: Marcus et al. (2010) Plant Journal 64, 191-203  ONLINE

Cat. No. LM22

MONOCLONAL ANTIBODY to MANNAN / LM22 

(Rat IgM)

SPECIFICITY: This antibody recognises β-linked mannan polysaccharides of plant cell walls. It has no known cross-reactivity with other polymers and can recognise heteromannan polysaccharides in several species. LM22 binds effectively to β-(1→4)-manno-oligosaccharides from DP2 to DP5. LM22 displays recognition of mannan and glucomannan polymers although the basis of its lack of recognition of galactomannan polysaccharides is not clear.   

REFERENCE: Marcus et al. (2010) Plant Journal 64, 191-203  ONLINE

Cat. No. LM7

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM7

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises a partially methyl-esterified epitope of homogalacturonan that results from non-blockwise de-esterification processes. It does not bind to un-esterified homogalacturonan.

REFERENCES: Willats et al. (2001) J. Biol. Chem 276, 19404-19413 ONLINE; Clausen et al. (2003) Carbohydr. Res. 338, 1797-1800. ONLINE

Cat. No. LM18

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM18

(Rat IgG2c)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody has some preference for partially methyl-esterified homogalacturonan but can also bind to un-esterified homogalacturonan.

REFERENCES: Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

Cat. No. LM19

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM19

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises a range of homogalacturonan samples but appears to have a preference for and binds strongly to un-esterified homogalacturonan. We now recommend the use of LM19 in the place of JIM5 as LM19 binds more effectively to unesterified HG.

REFERENCES: Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

Cat. No. LM20

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM20

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody requires methyl-esters for recognition of homogalacturonan and does not bind to un-esterified homogalacturonan. We now recommend the use of LM20 in the place of JIM7 as LM20 binding is more effectively lost by high pH treatments that remove HG methyl esters.

REFERENCES:  Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

Cat. No. JIM5

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / JIM5

(Rat IgG, JIM5)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises partially methyl-esterified epitopes of homogalacturonan and can also bind to un-esterified homogalacturonan.

We now recommend the use of LM19 in the place of JIM5 as LM19 binds more effectively to unesterified HG.

REFERENCES: Knox et al. (1990) Planta 181, 512-521; Willats et al. (2000) Carbohydr.  Res. 327, 309-320; Clausen et al. (2003) Carbohydr. Res. 338, 1797-1800 ONLINE; Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

Cat. No. JIM7

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / JIM7

(Rat IgA, JIM7)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises partially methyl-esterified epitopes of homogalacturonan but does not bind to un-esterified homogalacturonan.

We now recommend the use of LM20 in the place of JIM7 as LM20 binding is more effectively lost by high pH treatments that remove HG methyl esters.

REFERENCES: Knox et al. (1990) Planta 181, 512-521; Willats et al. (2000) Carbohydr.  Res. 327, 309-320; Clausen et al. (2003) Carbohydr. Res.  338, 1797-180 ONLINE; Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

Cat. No. LM8

MONOCLONAL ANTIBODY to XYLOGALACTURONAN / LM8

(Rat IgM, LM8)

SPECIFICITY: The antibody recognises a specific epitope of a xylogalacturonan pectic polysaccharide that is associated with cell detachment and separation in a wide range of species. It has no known cross-reactivity with other polymers. This antibody does NOT bind to all xylogalacturonans.

REFERENCE: Willats et al. (2004) Planta  218, 673-681. ONLINE

Cat. No. LM5

MONOCLONAL ANTIBODY to (1-4)-β-D-GALACTAN  / LM5

(Rat IgG)

SPECIFICITY: The antibody was generated using a neoglycoprotein (galactotetraose-BSA). It recognises a linear tetrasaccharide in (1-4)-β-D-galactans. It has no cross-reactivity with (1-3)-β-D-galactans or (1-6)-β-D-galactans. It can recognise pectic polysaccharides in several species. In competitive inhibition ELISA, antibody binding to (1-4)-β-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-β-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-β-D-galactan.

REFERENCE: Jones et al. (1997) Plant Physiol. 113, 1405-1412. ONLINE

Cat. No. LM9

MONOCLONAL ANTIBODY to FERULOYLATED-(1-4)-β-D-GALACTAN

(Rat IgM)

SPECIFICITY: The antibody recognises a specific epitope of a feruloylated-(1-4)-b-D-galactan that is a structural feature of the pectic polymers of plant species of the Amaranthaceae/Chenopodiaceae. It has no known cross-reactivity with other polymers. In competitive inhibition ELISAs, antibody binding to sugar beet pectin was inhibited (50%) by 15 µg/ml O-[6-O-(trans-feruloyl)-b-d-galactopyranosyl]-(1→4)-d-galactopyranose.

REFERENCE: Clausen et al. (2004) Planta  219, 1036-1041. ONLINE

Cat. No. LM6

MONOCLONAL ANTIBODY to (1-5)-a-L-ARABINAN / LM6

(Rat IgG)

SPECIFICITY: Generated using a neoglycoprotein (arabinoheptaose-BSA). Recognises a linear pentasaccharide in (1-5)-α-L-arabinans. It can recognise pectic polysaccharides in several species. It has no cross-reactivity with gum arabic but it may recognize arabinogalactan-proteins (AGPs) in some species. In competitive inhibition ELISAs, antibody binding to (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabino-hexaose.  

REFS: Willats et al. (1998) Carbohydr. Res. 308, 149-152 ONLINE; Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

Cat. No. LM13

MONOCLONAL ANTIBODY to (1-5)-a-L-ARABINAN (linear) / LM13

(Rat IgG)

SPECIFICITY: Isolated from a HTP screen of antibodies generated subsequent to immunization with a pectic fraction. Recognises a linear epitope in (1-5)-α-L-arabinans. Antibody recognition of arabinans increases with arabinofuranosidase action. This antibody binds to a specific subset of pectic arabinans, and to longer stretches of 1,5-linked arabinosyl residues that are likely to be more abundant in unbranched arabinans. The binding of LM13 is more sensitive to arabinanase action than LM6.

REFS: Moller et al. (2007) Glycoconjugate J. 25, 37-48; Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

Cat. No. LM16

MONOCLONAL ANTIBODY to PROCESSED ARABINAN - RG-I / LM16

(Rat IgM)

SPECIFICITY: Generated subsequent to immunization with a pectic fraction. Recognises a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues. The binding of LM16 is sensitive to galactosidase action and the epitope may involve galactosyl residue(s) on RG backbones.  

REFERENCE: Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

Cat. No. LM2

MONOCLONAL ANTIBODY to ARABINOGALACTAN-PROTEIN  / LM2

(Rat IgM)

SPECIFICITY: Generated to rice arabinogalactan-proteins (AGPs). Recognises a carbohydrate epitope containing b-linked glucuronic acid. It can recognise AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 µmg/ml 1-O-methyl-b-D-GlcA.  The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-b-D-GlcA.

REFERENCES: Smallwood et al. (1996) Planta 198, 452-459; Yates et al. (1996) Glycobiology 6, 131-139. ONLINE

Cat. No. LM14

MONOCLONAL ANTIBODY to ARABINOGALACTAN-PROTEIN  / LM14

(Rat IgM)

SPECIFICITY: Isolated from a HTP screen of antibodies generated subsequent to immunization with a pectic fraction. Recognizes arabinogalactan-proteins and will also bind to larch arabinogalactan. It can recognise AGPs in several species.

REFERENCE: Moller et al. (2007) Glycoconjugate J. 25, 37-48. ONLINE

Cat. No. LM1

MONOCLONAL ANTIBODY to EXTENSIN / LM1

(Rat IgM)

SPECIFICITY: Generated to rice extensin hydroxyproline-rich glycoproteins (HRGPs). The antibody recognises an epitope that is carried by a range of HRGPs of the extensin class in a wide range of angiosperm species. The LM1 epitope most likely includes extensin glycan components.

REFERENCE: Smallwood et al. (1995) Planta 196, 510-522.

 

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His-tagged recombinant proteins

Cat. No. PAM1

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / PAM1

(Phage display-derived scFv)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody is specific to unesterified homogalacturonan and recognises long un-esterified blocks of GalA residues.

REFERENCES: Willats et al. (1999) Plant Journal 18, 57-65; Willats et al. (2000) Carbohydrate Research 327, 309-320; Manfield et al. (2005) Plant Science 169, 1090-1095.

SUPPLY: PAM1 is provided as a his-tagged recombinant soluble scFv. PAM1 DATA SHEET

COST: £150 for 50 µg his-tagged PAM1 scFv.

Cat. No. CBM3a

CRYSTALLINE CELLULOSE-BINDING MODULE / CBM3a

(Recombinant CBM protein)

SPECIFICITY: This type A carbohydrate-binding module (CBM) from Clostridium thermocellum is directed to crystalline cellulose and can detect cellulose in both in vitro assays and directly in plant materials. Please note that this CBM can also bind to xyloglucan.

REFERENCES: Blake et al. (2006) J Biol. Chem. 281, 29321-29329. Hernandez-Gomez et al. (2015) FEBS Let. 589, 2297-2303

SUPPLY: CBM3a is provided as a his-tagged recombinant protein.

COST: £200 for 200 µg his-tagged CBM3a protein.

Mouse monoclonal antibody cell culture supernatant

 

Cat. No. 2F4

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / 2F4  

(Mouse IgG1)

SPECIFICITY: The 2F4 mouse monoclonal antibody recognises the homogalacturonan domain of pectic polysaccharides. It binds specifically to a dimeric association of pectic chains through calcium ions. It binds to homogalacturonans with degrees of methyl-esterification (DM) up to 40%.

REFERENCES: Liners et al. (1989) Plant Physiology 91, 1419-1424; Liners et al. (1992) Plant Physiology 99, 1099-1104; Liners & van Cutsem (1992) Protoplasma 170, 10-21.

SUPPLY: 2F4 is provided as a 1 mL volume of mouse hybridoma supernatant. 2F4 DATA SHEET

COST: £200 per 1 mL unit of 2F4 hybridoma supernatant.